Anti-ARIH2 Polyclonal Antibody (HD428014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HD428014

品牌

abinScience

描述

Anti-ARIH2 Polyclonal Antibody (HD428014) is a rabbit polyclonal antibody detecting ARIH2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ARIH2 (Thr62-Thr493).

靶标

ARI-2, ARI2, ARIH2, E3 ubiquitin-protein ligase ARIH2, EC:2.3.2.31, Protein ariadne-2 homolog, TRIAD1, Triad1 protein

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O95376

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

O95376

背景

E3 ubiquitin-protein ligase ARIH2 is a ~57 kDa protein. E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the cullin-5 (CUL5) component of the ECS complex. Together with the ECS(ASB9) complex, catalyzes ubiquitination of CKB.

1. Marteijn, JA. et al. (2005) Blood 106, 4114-23. PMID: 16118314

2. Marteijn, JA. et al. (2007) Blood 110, 3128-35. PMID: 17646546

3. Marteijn, JA. et al. (2009) Leukemia 23, 1480-9. PMID: 19340006

4. Kelsall, IR. et al. (2013) The EMBO journal 32, 2848-60. PMID: 24076655

5. Lumpkin, RJ. et al. (2021) Molecular & cellular proteomics : MCP 20, 100019. PMID: 33268465

6. Kostrhon, S. et al. (2021) Nature chemical biology 17, 1075-1083. PMID: 34518685

7. Xu, P. et al. (2024) Nature 627, 873-879. PMID: 38418882

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-ARIH2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HL-60 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate
Lane 5: Hela cell lysate

参考文献

产品说明书

Anti-ARIH2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ARIH2 Polyclonal Antibody (HD428014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-ARIH2 Polyclonal Antibody (HD428014)

概述

图片

参考文献

产品说明书

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