Anti-HAT1 Polyclonal Antibody (HD437014)

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50ug
100ug

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概述
图片
参考文献
产品说明书

概述

货号

HD437014

品牌

abinScience

描述

Anti-HAT1 Polyclonal Antibody (HD437014) is a rabbit polyclonal antibody detecting HAT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human HAT1 (Met1-Glu419).

靶标

EC:2.3.1.48, HAT1, Histone acetyltransferase 1, Histone acetyltransferase type B catalytic subunit, KAT1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O14929

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

O14929

背景

Histone acetyltransferase type B catalytic subunit (HAT1) is a ~49 kDa protein. Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair. Coordinates histone production and acetylation via H4 promoter binding. Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac). Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression.

1. Campos, EI. et al. (2010) Nature structural & molecular biology 17, 1343-51. PMID: 20953179

2. Yang, X. et al. (2013) The Journal of biological chemistry 288, 18271-82. PMID: 23653357

3. Gruber, JJ. et al. (2019) Molecular cell 75, 711-724.e5. PMID: 31278053

4. Agudelo Garcia, PA. et al. (2020) Journal of proteome research 19, 1663-1673. PMID: 32081014

5. Makowski, AM. et al. (2001) The Journal of biological chemistry 276, 43499-502. PMID: 11585814

6. Wu, H. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 8925-30. PMID: 22615379

7. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 31812350

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-HAT1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: MDA-MB-231 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293T cell lysate
Lane 5: Lncap cell lysate
Lane 6: K562 cell lysate
Lane 7: PC-12 cell lysate

参考文献

产品说明书

Anti-HAT1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAT1 Polyclonal Antibody (HD437014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-HAT1 Polyclonal Antibody (HD437014)

概述

图片

参考文献

产品说明书

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