Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名
P31939
背景
Bifunctional purine biosynthesis protein ATIC is a ~64 kDa protein. Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction. Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias.
1. Bulock, KG. et al. (2002) The Journal of biological chemistry 277, 22168-74. PMID: 11948179
2. Vergis, JM. et al. (2004) Biochemistry 43, 1184-92. PMID: 14756554
3. Wall, M. et al. (2000) Biochemistry 39, 11303-11. PMID: 10985775
4. Sugita, T. et al. (1997) Journal of biochemistry 122, 309-13. PMID: 9378707
5. Boutchueng-Djidjou, M. et al. (2015) Molecular & cellular proteomics : MCP 14, 1079-92. PMID: 25687571
Note
For research use only
图片
Western blot
Western blot analysis was performed using anti-ATIC polyclonal antibody at 1ug/mL on various samples. Lane 1: 293T cell lysate Lane 2: Hela cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: HCT116 cell lysate Lane 6: A549 cell lysate Lane 7: MCF-7 cell lysate Lane 8: HT-29 cell lysate Lane 9: K562 cell lysate Lane 10: NIH3T3 cell lysate Lane11: PC-12 cell lysate
