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Anti-PEPD Polyclonal Antibody (HD441014)

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规格:
  • 50ug
  • 100ug
数量:
  • 概述

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  • 参考文献

  • 产品说明书

概述
货号HD441014
品牌abinScience
描述
Anti-PEPD Polyclonal Antibody (HD441014) is a rabbit polyclonal antibody detecting PEPD in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human PEPD (Thr188-Lys493).
靶标EC:3.4.13.9, Imidodipeptidase, PEPD, PRD, Peptidase D, Prolidase, Proline dipeptidase, X-Pro dipeptidase, Xaa-Pro dipeptidase
内毒素水平Please contact with the lab for this information.
纯化方式Purified by antigen affinity column.
Accession号P12955
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息
应用方法 稀释比例
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
别名P12955
背景

Xaa-Pro dipeptidase (PEPD) is a ~54 kDa protein. Dipeptidase that catalyzes the hydrolysis of dipeptides with a prolyl (Xaa-Pro) or hydroxyprolyl residue in the C-terminal position. The preferred dipeptide substrate is Gly-Pro, but other Xaa-Pro dipeptides, such as Ala-Pro, Met-Pro, Phe-Pro, Val-Pro and Leu-Pro, can be cleaved. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.

1. Lupi, A. et al. (2006) The FEBS journal 273, 5466-78. PMID: 17081196
2. Rao, SD. et al. (2022) Nature chemical biology 18, 565-574. PMID: 35165443
3. Endo, F. et al. (1989) The Journal of biological chemistry 264, 4476-81. PMID: 2925654
NoteFor research use only
图片
  • Western blot

    Western blot analysis was performed using anti-PEPD polyclonal antibody at 1ug/mL on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: SH-SY5Y cell lysate

参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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