Anti-CCT5 Polyclonal Antibody (HW730014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

HW730014

品牌

abinScience

描述

Anti-CCT5 Polyclonal Antibody (HW730014) is a rabbit polyclonal antibody detecting CCT5 in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

同种型

IgG

克隆类型

Polyclonal

免疫原

E. coli - derived recombinant Human CCT5 (Met1-Glu541).

靶标

CCT-epsilon, CCT5, CCTE, Chaperonin containing T-complex polypeptide 1 subunit 5, EC:3.6.1.-, KIAA0098, T-complex protein 1 subunit epsilon, TCP-1-epsilon

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P48643

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

别名

P48643

背景

T-complex protein 1 subunit epsilon (CCT5) is a ~59 kDa protein. Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.

1. Freund, A. et al. (2014) Cell 159, 1389-403. PMID: 25467444

2. Gestaut, D. et al. (2022) Cell 185, 4770-4787.e20. PMID: 36493755

3. Kelly, JJ. et al. (2022) Nature structural & molecular biology 29, 420-429. PMID: 35449234

4. Liu, C. et al. (2023) Communications biology 6, 531. PMID: 37193829

5. Seo, S. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 1488-93. PMID: 20080638

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-CCT5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: A431 cell lysate
Lane 3: 293T cell lysate
Lane 4: MOLT-4 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: HepG2 cell lysate
Lane 7: NIH3T3 cell lysate
Lane 8: A549 cell lysate

参考文献

产品说明书

Anti-CCT5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CCT5 Polyclonal Antibody (HW730014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-CCT5 Polyclonal Antibody (HW730014)

概述

图片

参考文献

产品说明书

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