Anti-MSUN2 Polyclonal Antibody (MC621014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

MC621014

品牌

abinScience

描述

Anti-MSUN2 Polyclonal Antibody (MC621014) is a rabbit polyclonal antibody detecting MSUN2 in ELISA, IHC, WB. Suitable for Mouse and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant mouse MSUN2 (Asn45-Ala431).

靶标

D13Wsu123e, EC:2.1.1.-, EC:2.1.1.203, Misu, Myc-induced SUN domain-containing protein, NOL1/NOP2/Sun domain family member 2, Nsun2, RNA cytosine C(5)-methyltransferase NSUN2, mRNA cytosine C(5)-methyltransferase, tRNA cytosine C(5)-methyltransferase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q1HFZ0

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

RNA cytosine C(5)-methyltransferase NSUN2 (MSUN2) is a ~85 kDa protein. RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs.

1. Blanco, S. et al. (2011) PLoS genetics 7, e1002403. PMID: 22144916

2. Hussain, S. et al. (2013) Cell reports 4, 255-61. PMID: 23871666

3. Gkatza, NA. et al. (2019) PLoS biology 17, e3000297. PMID: 31199786

4. Tuorto, F. et al. (2012) Nature structural & molecular biology 19, 900-5. PMID: 22885326

5. Hussain, S. et al. (2013) Molecular and cellular biology 33, 1561-70. PMID: 23401851

6. Van Haute, L. et al. (2019) Nucleic acids research 47, 8720-8733. PMID: 31276587

Note

For research use only

图片

参考文献

产品说明书

Anti-MSUN2 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MSUN2 Polyclonal Antibody (MC621014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-MSUN2 Polyclonal Antibody (MC621014)

概述

图片

参考文献

产品说明书

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