Anti-GETV Spike glycoprotein E1 Polyclonal Antibody (VK157014)

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概述
图片
参考文献
产品说明书

概述

货号

VK157014

品牌

abinScience

描述

Anti-GETV Spike glycoprotein E1 Polyclonal Antibody (VK157014) is a rabbit polyclonal antibody detecting Spike glycoprotein E1 in ELISA, IHC, WB. Suitable for Getah virus, Sagiyama virus, and Ross river virus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

种属反应性

Getah virus (GETV), Ross River virus (RRV)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant GETV Structural polyprotein (Val826-Cys1191).

靶标

6K protein, Assembly protein E3, C, Capsid protein, Coat protein, E1 envelope glycoprotein, E2 envelope glycoprotein, EC:3.4.21.90, Precursor of protein E3/E2, Spike glycoprotein E1, Spike glycoprotein E2, Structural polyprotein, p130, p62, pE2

内毒素水平

Please contact with the lab for this information.

观测分子量

Q9JGK8,P13890,P08491

纯化方式

Purified by antigen affinity column.

Accession号

Q5Y388

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

稳定性和存储

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Structural polyprotein is a ~137 kDa protein. Forms an icosahedral capsid with a T=4 symmetry composed of 240 copies of the capsid protein surrounded by a lipid membrane through which penetrate 80 spikes composed of trimers of E1-E2 heterodimers. The capsid protein binds to the viral RNA genome at a site adjacent to a ribosome binding site for viral genome translation following genome release. Possesses a protease activity that results in its autocatalytic cleavage from the nascent structural protein. Following its self-cleavage, the capsid protein transiently associates with ribosomes, and within several minutes the protein binds to viral RNA and rapidly assembles into icosahedric core particles. The resulting nucleocapsid eventually associates with the cytoplasmic domain of the spike glycoprotein E2 at the cell membrane, leading to budding and formation of mature virions.

1. Wang, A. et al. (2022) Cell discovery 8, 12. PMID: 35149682

Note

For research use only

图片

参考文献

产品说明书

Anti-GETV Spike glycoprotein E1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GETV Spike glycoprotein E1 Polyclonal Antibody (VK157014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-GETV Spike glycoprotein E1 Polyclonal Antibody (VK157014)

概述

图片

参考文献

产品说明书

相关产品推荐

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