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InVivoMAb Anti-Rotavirus A/RV-A Outer capsid glycoprotein VP7 (Iv0011) (ARO-A13331)

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概述
货号ARO-A13331
品牌ProteoGenix
描述
InVivoMAb Anti-Rotavirus A/RV-A Outer capsid glycoprotein VP7 (Iv0011) (ARO-A13331) Purity: >95% as determined by SDS-PAGE..
Highlights
  • Protein A/G purified from cell culture supernatant. — Ensuring high specificity and minimal background.
  • Multi-Application — Validated across multiple applications.
种属反应性Rotavirus A (strain RVA/Monkey/United States/RRV/1975/G3P5B[3]) (RV-A)
应用ELISA, Neutralization
宿主Mouse
同种型IgG2a, kappa
克隆号Iv0011
克隆类型Monoclonal
靶标Outer capsid glycoprotein VP7
内毒素水平Please contact with the lab for this information.
纯度>95% as determined by SDS-PAGE.
相关产品 pH 7.4 Dilution Buffer
纯化方式Protein A/G purified from cell culture supernatant.
Accession号P12476
状态Liquid
保存溶液 0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

稳定性和存储Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景

Outer capsid glycoprotein VP7 is a ~37 kDa protein. Calcium-binding protein that interacts with rotavirus cell receptors once the initial attachment by VP4 has been achieved. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. Following entry into the host cell, low intracellular or intravesicular Ca(2+) concentration probably causes the calcium-stabilized VP7 trimers to dissociate from the virion. This step is probably necessary for the membrane-disrupting entry step and the release of VP4, which is locked onto the virion by VP7.

1. Aoki, ST. et al. (2011) Journal of virology 85, 10509-17. PMID: 21849465
2. Abdelhakim, AH. et al. (2014) PLoS pathogens 10, e1004355. PMID: 25211455
3. Settembre, EC. et al. (2011) The EMBO journal 30, 408-16. PMID: 21157433
NoteFor research use only. Not suitable for clinical or therapeutic use.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量,自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项,自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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