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Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (ARO-A12726)

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概述
货号ARO-A12726
品牌ProteoGenix
描述
Anti-Bacillus licheniformis BliGO/thiO Polyclonal Antibody (ARO-A12726) is a rabbit polyclonal antibody detecting Glycine oxidase, GO, 1.4.3.19, BliGO, thiO in ELISA, IHC, WB. Suitable for Bacillus licheniformis.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Bacillus licheniformis
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Bacillus licheniformis BliGO/thiO (Met1-Arg369).
靶标Glycine oxidase, GO, 1.4.3.19, BliGO, thiO
纯化方式Purified by antigen affinity column.
Accession号S5FMM4
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Glycine oxidase (BliGO/thiO) is a ~40 kDa protein. Catalyzes the FAD-dependent oxidative deamination of glycine, leading to glyoxylate, ammonia and hydrogen peroxide. Is also able to act on various amines and D-amino acids to yield the corresponding alpha-keto acids, ammonia/amine, and hydrogen peroxide. Can also oxidize the herbicide glyphosate (N-phosphonomethylglycine), and thus may be involved in the degradation pathway that allows B.licheniformis J33-8 to grow with glyphosate as the sole source of carbon. Is essential for thiamine biosynthesis since the oxidation of glycine catalyzed by ThiO generates the glycine imine intermediate (dehydroglycine) required for the biosynthesis of the thiazole ring of thiamine pyrophosphate.

1. Zhang, K. et al. (2016) Enzyme and microbial technology 85, 12-8. PMID: 26920475
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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