Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Dynamin-like GTPase OPA1, mitochondrial is a ~111 kDa protein. Dynamin-related GTPase that is essential for normal mitochondrial morphology by mediating fusion of the mitochondrial inner membranes, regulating cristae morphology and maintaining respiratory chain function. Exists in two forms: the transmembrane, long form (Dynamin-like GTPase OPA1, long form; L-OPA1), which is tethered to the inner mitochondrial membrane, and the short soluble form (Dynamin-like GTPase OPA1, short form; S-OPA1), which results from proteolytic cleavage and localizes in the intermembrane space. Both forms (L-OPA1 and S-OPA1) cooperate to catalyze the fusion of the mitochondrial inner membrane. The equilibrium between L-OPA1 and S-OPA1 is essential: excess levels of S-OPA1, produced by cleavage by OMA1 following loss of mitochondrial membrane potential, lead to an impaired equilibrium between L-OPA1 and S-OPA1, inhibiting mitochondrial fusion. The balance between L-OPA1 and S-OPA1 also influences cristae shape and morphology.
1. Ishihara, N. et al. (2006) The EMBO journal 25, 2966-77. PMID: 16778770
2. Song, Z. et al. (2007) The Journal of cell biology 178, 749-55. PMID: 17709429
3. Ban, T. et al. (2010) Human molecular genetics 19, 2113-22. PMID: 20185555
4. Anand, R. et al. (2014) The Journal of cell biology 204, 919-29. PMID: 24616225
5. Ban, T. et al. (2017) Nature cell biology 19, 856-863. PMID: 28628083
6. Huang, G. et al. (2017) Cell reports 20, 923-934. PMID: 28746876
