| 背景 | Pre-mRNA-processing-splicing factor 8 (PRPF8) is a ~273 kDa protein. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site. 1. Luo, HR. et al. (1999) RNA (New York, N.Y.) 5, 893-908. PMID: 104111332. Schneider, C. et al. (2002) Molecular and cellular biology 22, 3219-29. PMID: 119719556. Zhan, X. et al. (2018) Science (New York, N.Y.) 359, 537-545. PMID: 2930196110. Fica, SM. et al. (2019) Science (New York, N.Y.) 363, 710-714. PMID: 30705154 |
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