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Anti-Human RNF20 Polyclonal Antibody (ARO-A11997)

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概述
货号ARO-A11997
品牌ProteoGenix
描述
Anti-Human RNF20 Polyclonal Antibody (ARO-A11997) is a rabbit polyclonal antibody detecting RING-type E3 ubiquitin transferase BRE1A, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A, RNF20, hBRE1, RING finger protein 20 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human RNF20 (Asp906-Gly975).
靶标RING-type E3 ubiquitin transferase BRE1A, E3 ubiquitin-protein ligase BRE1A, BRE1-A, BRE1A, RNF20, hBRE1, RING finger protein 20
纯化方式Purified by antigen affinity column.
Accession号Q5VTR2
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

E3 ubiquitin-protein ligase BRE1A (RNF20) is a ~113 kDa protein. Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.

NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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