Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Splicing factor 3B subunit 3 (SF3B3) is a ~135 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B3 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
1. Das, BK. et al. (1999) Molecular and cellular biology 19, 6796-802. PMID: 10490618
2. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114
3. Will, CL. et al. (2002) The EMBO journal 21, 4978-88. PMID: 12234937
4. Cretu, C. et al. (2016) Molecular cell 64, 307-319. PMID: 27720643
5. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166
6. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006
7. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310
