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Anti-Human TPK1 Polyclonal Antibody (ARO-A12367)

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概述
货号ARO-A12367
品牌ProteoGenix
描述
Anti-Human TPK1 Polyclonal Antibody (ARO-A12367) is a rabbit polyclonal antibody detecting hTPK1, Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, TPK1, PP20, Placental protein 20 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human TPK1 (Met1-Ser243).
靶标hTPK1, Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, TPK1, PP20, Placental protein 20
纯化方式Purified by antigen affinity column.
Accession号Q9H3S4
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Thiamine pyrophosphokinase 1 (TPK1) is a ~27 kDa protein. Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP) utilizing UTP and therefore links the biosynthesis of TPP to pyrimidines metabolism. By producing thiamine pyrophosphate, a cofactor of the mitochondrial pyruvate dehydrogenase indirectly regulates pyruvate oxidation and lipogenesis. Although it can also catalyze thiamine phosphorylation using ATP and CTP in vitro, it does so with significantly lower efficiency and without physiological relevance evidence.

1. Sahu, U. et al. (2024) Science (New York, N.Y.) 383, 1484-1492. PMID: 38547260
2. Nosaka, K. et al. (2001) Biochimica et biophysica acta 1517, 293-7. PMID: 11342111
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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