Affinity Purified — Minimal background and high purity for reliable results.
Multi-Application — Validated across multiple applications.
种属反应性
Human
应用
ELISA, IHC, WB
宿主
Rabbit
同种型
IgG
克隆类型
Polyclonal
免疫原
E. coli - derived recombinant Human UHRF1 (Leu134-Asp366).
靶标
Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, RING finger protein 106, Transcription factor ICBP90, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, NP95, HuNp95, Nuclear protein 95, RNF106, ICBP90, hNp95, UHRF1, Inverted CCAAT box-binding protein of 90 kDa, hUHRF1, Ubiquitin-like-containing PHD and RING finger domains protein 1
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
E3 ubiquitin-protein ligase UHRF1 is a ~89 kDa protein. E3 ubiquitin-protein ligase that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Plays a key role in DNA methylation inheritance by promoting recruitment of DNMT1 to hemimethylated DNA and ensure faithful propagation of the DNA methylation patterns through DNA replication. Acts both as a histone reader and writer: specifically recognizes and binds (1) hemimethylated DNA at replication forks and (2) histone H3 trimethylated at 'Lys-9' and unmethylated at 'Arg-2' (H3K9me3 and H3R2me0, respectively), thereby activating its E3 ubiquitin-protein ligase activity. UHRF1 then mediates histone H3 'Lys-18' monoubiquitination (H3K18ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Also mediates histone H3 'Lys-14' and 'Lys-23' ubiquitination (H3K14ub and H3K23ub) at lower level.
1. Hopfner, R. et al. (2000) Cancer research 60, 121-8. PMID: 10646863
2. Arima, Y. et al. (2004) Genes to cells : devoted to molecular & cellular mechanisms 9, 131-42. PMID: 15009091
3. Kim, JK. et al. (2009) Nucleic acids research 37, 493-505. PMID: 19056828
4. Rothbart, SB. et al. (2012) Nature structural & molecular biology 19, 1155-60. PMID: 23022729
5. Nishiyama, A. et al. (2013) Nature 502, 249-53. PMID: 24013172
