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Anti-Human UBE2T Polyclonal Antibody (ARO-A12457)

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概述
货号ARO-A12457
品牌ProteoGenix
描述
Anti-Human UBE2T Polyclonal Antibody (ARO-A12457) is a rabbit polyclonal antibody detecting Ubiquitin-protein ligase T, Ubiquitin carrier protein T, UBE2T, Cell proliferation-inducing gene 50 protein, Ubiquitin-conjugating enzyme E2 T, E2 ubiquitin-conjugating enzyme T in ELISA, IHC, WB. Suitable for Bos taurus, Human, Mouse.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Bos taurus, Human, Mouse
应用ELISA, IHC, WB
宿主Rabbit
克隆类型Polyclonal
同种型IgG
免疫原E. coli - derived recombinant Human UBE2T (Met1-Arg152).
靶标Ubiquitin-protein ligase T, Ubiquitin carrier protein T, UBE2T, Cell proliferation-inducing gene 50 protein, Ubiquitin-conjugating enzyme E2 T, E2 ubiquitin-conjugating enzyme T
纯化方式Purified by antigen affinity column.
Accession号Q9NPD8
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Ubiquitin-conjugating enzyme E2 T (UBE2T) is a ~22 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI.

1. Machida, YJ. et al. (2006) Molecular cell 23, 589-96. PMID: 16916645
2. Alpi, A. et al. (2007) Molecular and cellular biology 27, 8421-30. PMID: 17938197
3. Alpi, AF. et al. (2008) Molecular cell 32, 767-77. PMID: 19111657
4. Longerich, S. et al. (2009) The Journal of biological chemistry 284, 23182-6. PMID: 19589784
5. Morreale, FE. et al. (2017) Journal of medicinal chemistry 60, 4093-4098. PMID: 28437106
6. Ueki, T. et al. (2009) Cancer research 69, 8752-60. PMID: 19887602
7. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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