E. coli - derived recombinant Human CHMP2A (Pro11-Asn181).
靶标
Vps2-1, Putative breast adenocarcinoma marker BC-2, CHMP2A, hVps2-1, CHMP2a, Vacuolar protein sorting-associated protein 2-1, Charged multivesicular body protein 2a, CHMP2, BC2, Chromatin-modifying protein 2a
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Charged multivesicular body protein 2a (CHMP2A) is a ~25 kDa protein. Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis.
1. Guizetti, J. et al. (2011) Science (New York, N.Y.) 331, 1616-20. PMID: 21310966
2. Vietri, M. et al. (2015) Nature 522, 231-5. PMID: 26040712
3. Gu, M. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E2166-E2175. PMID: 28242692
