Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Ribosomal protein S6 kinase beta-1 (S6K1/RPS6KB1) is a ~59 kDa protein. Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mechanistic target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B.
1. Wang, X. et al. (2001) The EMBO journal 20, 4370-9. PMID: 11500364
2. Fleckenstein, DS. et al. (2003) Leukemia research 27, 687-94. PMID: 12801526
3. Fingar, DC. et al. (2004) Molecular and cellular biology 24, 200-16. PMID: 14673156
4. Raught, B. et al. (2004) The EMBO journal 23, 1761-9. PMID: 15071500
5. Richardson, CJ. et al. (2004) Current biology : CB 14, 1540-9. PMID: 15341740
