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Anti-PSAT1 Polyclonal Antibody (ARO-A13571)

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概述
货号ARO-A13571
品牌ProteoGenix
描述
Anti-PSAT1 Polyclonal Antibody (ARO-A13571) is a rabbit polyclonal antibody detecting Phosphohydroxythreonine aminotransferase, PSAT, PSAT1, PSA, Phosphoserine aminotransferase in ELISA, IHC, WB. Suitable for Human, Mouse, Oryctolagus cuniculus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Oryctolagus cuniculus
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human PSAT1 (Met1-Gly312).
靶标Phosphohydroxythreonine aminotransferase, PSAT, PSAT1, PSA, Phosphoserine aminotransferase
纯化方式Purified by antigen affinity column.
Accession号Q9Y617
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Phosphoserine aminotransferase (PSAT1) is a ~40 kDa protein. Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate. Acts as an inhibitor of ferroptosis in response to interferon-gamma (IFNG) by promoting GPX4 stability: following phosphorylation by CAMK2A, PSAT1 interacts with GPX4 and provides 2-oxoglutarate to EGLN3, leading to GPX4 hydroxylation and stability.

1. Marchesani, F. et al. (2023) Protein science : a publication of the Protein Society 32, e4609. PMID: 36851825
2. Marchesani, F. et al. (2023) Biomolecules 13. PMID: 37627284
3. Zheng, P. et al. (2025) Nature chemical biology 21, 1420-1432. PMID: 40281343
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Anti-PSAT1 Polyclonal Antibody

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