Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Spindlin-1 (SPIN1) is a ~29 kDa protein. Chromatin reader that specifically recognizes and binds histone H3 both trimethylated at 'Lys-4' and 'Lys-9' (H3K4me3K9me3) and is involved in piRNA-mediated retrotransposon silencing during spermatogenesis. Plays a key role in the initiation of the PIWIL4-piRNA pathway, a pathway that directs transposon DNA methylation and silencing in the male embryonic germ cells, by promoting recruitment of DNA methylation machinery to transposons: binds young, but not old, LINE1 transposons, which are specifically marked with H3K4me3K9me3, and promotes the recruitment of PIWIL4 and SPOCD1 to transposons, leading to piRNA-directed DNA methylation. Also recognizes and binds histone H3 both trimethylated at 'Lys-4' and asymmetrically dimethylated at 'Arg-8' (H3K4me3 and H3R8me2a) and acts as an activator of Wnt signaling pathway downstream of PRMT2. In case of cancer, promotes cell cancer proliferation via activation of the Wnt signaling pathway. Overexpression induces metaphase arrest and chromosomal instability.
1. Du, Y. et al. (2021) Nature communications 12, 949. PMID: 33574238
2. Wang, JX. et al. (2012) Molecular cancer research : MCR 10, 326-35. PMID: 22258766
3. Bae, N. et al. (2017) The Journal of biological chemistry 292, 20808-20817. PMID: 29061846
4. Su, X. et al. (2014) Genes & development 28, 622-36. PMID: 24589551
5. Wang, W. et al. (2011) EMBO reports 12, 1160-6. PMID: 21960006
