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Anti-PPIE Polyclonal Antibody (ARO-A13596)

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概述
货号ARO-A13596
品牌ProteoGenix
描述
Anti-PPIE Polyclonal Antibody (ARO-A13596) is a rabbit polyclonal antibody detecting Peptidyl-prolyl cis-trans isomerase E, PPIE, PPIase E, Cyclophilin-33, CYP33, Rotamase E, Cyclophilin E in ELISA, IHC, WB. Suitable for Bos taurus, Human, Mouse, Pongo abelii.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Bos taurus, Human, Mouse, Pongo abelii
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human PPIE (Tyr41-Val264).
靶标Peptidyl-prolyl cis-trans isomerase E, PPIE, PPIase E, Cyclophilin-33, CYP33, Rotamase E, Cyclophilin E
纯化方式Purified by antigen affinity column.
Accession号Q9UNP9
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Peptidyl-prolyl cis-trans isomerase E (PPIE) is a ~33 kDa protein. Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.

1. Jurica, MS. et al. (2002) RNA (New York, N.Y.) 8, 426-39. PMID: 11991638
2. Bertram, K. et al. (2017) Nature 542, 318-323. PMID: 28076346
3. Wang, Y. et al. (2008) FEBS letters 582, 835-9. PMID: 18258190
4. Hom, RA. et al. (2010) Journal of molecular biology 400, 145-54. PMID: 20460131
5. Park, S. et al. (2010) Biochemistry 49, 6576-86. PMID: 20677832
6. Mi, H. et al. (1996) FEBS letters 398, 201-5. PMID: 8977107
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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