| 背景 | DNA repair protein RAD50 is a ~153 kDa protein. Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. The MRN complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11, to initiate end resection, which is required for single-strand invasion and recombination. Within the complex, RAD50 is both required to bind DNA ends and hold them in close proximity and regulate the activity of MRE11. 1. Lee, JH. et al. (2004) Science (New York, N.Y.) 304, 93-6. PMID: 150644162. Gatei, M. et al. (2011) The Journal of biological chemistry 286, 31542-56. PMID: 217577804. Park, YB. et al. (2017) Nature structural & molecular biology 24, 248-257. PMID: 281349325. Myler, LR. et al. (2017) Molecular cell 67, 891-898.e4. PMID: 288672927. Paull, TT. et al. (1998) Molecular cell 1, 969-79. PMID: 96515808. Trujillo, KM. et al. (1998) The Journal of biological chemistry 273, 21447-50. PMID: 97052719. de Jager, M. et al. (2001) Molecular cell 8, 1129-35. PMID: 11741547 |
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