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Anti-NCF2 Polyclonal Antibody (ARO-A14072)

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概述
货号ARO-A14072
品牌ProteoGenix
描述
Anti-NCF2 Polyclonal Antibody (ARO-A14072) is a rabbit polyclonal antibody detecting Neutrophil NADPH oxidase factor 2, NOXA2, p67-phox, NADPH oxidase activator 2, 67 kDa neutrophil oxidase factor, Neutrophil cytosol factor 2, NCF-2, NCF2, P67PHOX in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human NCF2 (Lys355-Val526).
靶标Neutrophil NADPH oxidase factor 2, NOXA2, p67-phox, NADPH oxidase activator 2, 67 kDa neutrophil oxidase factor, Neutrophil cytosol factor 2, NCF-2, NCF2, P67PHOX
纯化方式Purified by antigen affinity column.
Accession号P19878
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Neutrophil cytosol factor 2 (NCF2) is a ~59 kDa protein. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox.

1. Yoshida, L. et al. (2002) Biochemical and biophysical research communications 296, 1322-8. PMID: 12207919
2. Liu, X. et al. (2024) Nature 627, 189-195. PMID: 38355798
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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