Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Aldo-keto reductase family 1 member C4 (AKR1C4) is a ~37 kDa protein. Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 3alpha/beta-, 17beta- and 20alpha-hydroxysteroids. Required for male sex determination as a component of the 'backdoor' androgen biosynthesis pathway that generates 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes. Acts together with AKR1C2 to convert 5alpha-dihydroprogesterone (5alpha-DHP) to 3alpha-hydroxy-5alpha-pregnan-20-one (3alpha,5alpha-THP/allopregnanolone), leading to 5alpha-DHT secretion necessary for embryonic gonad differentiation into testis. May regulate the concentrations of circulating neurosteroids.
1. Kume, T. et al. (1999) Pharmacogenetics 9, 763-71. PMID: 10634139
2. Penning, TM. et al. (2000) The Biochemical journal 351, 67-77. PMID: 10998348
3. Dufort, I. et al. (2001) The Journal of clinical endocrinology and metabolism 86, 841-6. PMID: 11158055
4. Steckelbroeck, S. et al. (2004) The Journal of biological chemistry 279, 10784-95. PMID: 14672942
5. Binstock, JM. et al. (1992) Biochemical and biophysical research communications 187, 760-6. PMID: 1530633
6. Higaki, Y. et al. (2003) Chemico-biological interactions 143-144, 503-13. PMID: 12604236
7. Jin, Y. et al. (2009) The Journal of biological chemistry 284, 10013-22. PMID: 19218247
8. Flück, CE. et al. (2011) American journal of human genetics 89, 201-18. PMID: 21802064
9. Khanna, M. et al. (1995) The Journal of biological chemistry 270, 20162-8. PMID: 7650035
10. Endo, S. et al. (2020) The Journal of steroid biochemistry and molecular biology 199, 105586. PMID: 31926269
