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Anti-NPM1 Polyclonal Antibody (ARO-A14204)

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概述
货号ARO-A14204
品牌ProteoGenix
描述
Anti-NPM1 Polyclonal Antibody (ARO-A14204) is a rabbit polyclonal antibody detecting NPM, Nucleolar protein NO38, Numatrin, NPM1, Nucleolar phosphoprotein B23, Nucleophosmin in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bos taurus.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
种属反应性Human, Mouse, Rat, Bos taurus
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human NPM1 (Gly20-Lys154).
靶标NPM, Nucleolar protein NO38, Numatrin, NPM1, Nucleolar phosphoprotein B23, Nucleophosmin
纯化方式Purified by antigen affinity column.
Accession号P06748
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleophosmin (NPM1) is a ~32 kDa protein. Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA.

1. Liu, X. et al. (2012) The Journal of biological chemistry 287, 19599-609. PMID: 22528486
2. Kim, JY. et al. (2015) The American journal of pathology 185, 2061-8. PMID: 25956029
NoteFor research use only.
图片
参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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