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Anti-HEXB Polyclonal Antibody (ARO-A14190)

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概述
货号ARO-A14190
品牌ProteoGenix
描述
Anti-HEXB Polyclonal Antibody (ARO-A14190) is a rabbit polyclonal antibody detecting N-Acetyl-β-glucosaminidase (NAG), chitobiase, NAG, Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, N-acetyl-beta-glucosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, Hexosaminidase subunit B, HCC-7, HEXB in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
种属反应性Human
应用ELISA, IHC, WB
宿主Rabbit
同种型IgG
克隆类型Polyclonal
免疫原E. coli - derived recombinant Human HEXB (Lys315-Tyr456).
靶标N-Acetyl-β-glucosaminidase (NAG), chitobiase, NAG, Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, N-acetyl-beta-glucosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, Hexosaminidase subunit B, HCC-7, HEXB
纯化方式Purified by antigen affinity column.
Accession号P07686
状态Liquid
保存溶液 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
稳定性和存储Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Beta-hexosaminidase subunit beta (HEXB/HCC7) is a ~63 kDa protein. Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.

1. Hepbildikler, ST. et al. (2002) The Journal of biological chemistry 277, 2562-72. PMID: 11707436
2. Novak, A. et al. (1994) Biochimica et biophysica acta 1199, 215-23. PMID: 8123671
3. Hou, Y. et al. (1996) Biochemistry 35, 3963-9. PMID: 8672428
4. Hou, Y. et al. (1998) The Journal of biological chemistry 273, 21386-92. PMID: 9694901
NoteFor research use only.
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参考文献
公式
质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)
填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。
质量
=
浓度
×
体积
分子量 *
g/mol
公式
C₁ × V₁ = C₂ × V₂
填写 4 项中的任意 3 项，自动计算未知值。
母液
C₁ (起始浓度)
×
V₁ (起始体积)
=
工作液
C₂ (终浓度)
×
V₂ (终体积)

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Anti-HEXB Polyclonal Antibody

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