Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
重悬
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
产品使用信息
应用方法
稀释比例
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景
Lysosomal acid lipase/cholesteryl ester hydrolase is a ~45 kDa protein. Catalyzes the deacylation of cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol. Hydrolyzes triglycerides (1,2,3-triacylglycerol) and diglycerides (such as 1,2-diacylglycerol and 1,3-diacylglycerol) with preference for the acyl moieties at the sn-1 or sn-3 positions.
1. Zschenker, O. et al. (2004) Journal of biochemistry 136, 65-72. PMID: 15269241
2. Anderson, RA. et al. (1991) The Journal of biological chemistry 266, 22479-84. PMID: 1718995
3. Warner, TG. et al. (1981) The Journal of biological chemistry 256, 2952-7. PMID: 7204383
4. Ameis, D. et al. (1994) European journal of biochemistry 219, 905-14. PMID: 8112342
5. Ries, S. et al. (1998) Human mutation 12, 44-51. PMID: 9633819
