Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody (ZA998014)

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概述
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产品说明书

概述

货号

ZA998014

品牌

abinScience

描述

Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody (ZA998014) is a rabbit polyclonal antibody detecting Renilla-type luciferase. Suitable for Sea pansy.

种属反应性

Renilla reniformis (Sea pansy)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Renilla reniformis Rluc/Luciferase (Met1-Gln311).

靶标

Coelenterazine h 2-monooxygenase, EC:1.13.12.5, Renilla-luciferin 2-monooxygenase, Renilla-type luciferase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P27652

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Coelenterazine h 2-monooxygenase (Rluc/Luciferase) is a ~36 kDa protein. Upon binding the substrate, the enzyme catalyzes an oxygenation, producing a very short-lived hydroperoxide that cyclizes into a dioxetanone structure, which collapses, releasing a CO(2) molecule. The spontaneous breakdown of the dioxetanone releases the energy (about 50 kcal/mole) that is necessary to generate the excited state of the coelenteramide product, which is the singlet form of the monoanion. In vivo the product undergoes the process of nonradiative energy transfer to an accessory protein, a green fluorescent protein (GFP), which results in green bioluminescence. In vitro, in the absence of GFP, the product emits blue light.

Note

For research use only

图片

参考文献

产品说明书

Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody (ZA998014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Renilla reniformis Rluc/Luciferase Polyclonal Antibody (ZA998014)

概述

图片

参考文献

产品说明书

相关产品推荐

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