Anti-Human CYP1B1 Polyclonal Antibody (HA565014)

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50ug
100ug

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概述
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参考文献
产品说明书

概述

货号

HA565014

品牌

abinScience

描述

Anti-Human CYP1B1 Polyclonal Antibody (HA565014) is a rabbit polyclonal antibody detecting CYP1B1. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CYP1B1 (Leu224-Asp492).

靶标

CYP1B1, CYPIB1, Cytochrome P450 1B1, EC:1.14.14.1, EC:4.2.1.152, Hydroperoxy icosatetraenoate dehydratase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q16678

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Cytochrome P450 1B1 (CYP1B1) is a ~60 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites.

1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376

2. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828

3. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317

4. Choudhary, D. et al. (2004) Drug metabolism and disposition: the biological fate of chemicals 32, 840-7. PMID: 15258110

5. Mesaros, C. et al. (2010) Rapid communications in mass spectrometry : RCM 24, 3237-47. PMID: 20972997

Note

For research use only

图片

Western blot

Lane 1: Hela cell lysate

参考文献

产品说明书

Anti-Human CYP1B1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CYP1B1 Polyclonal Antibody (HA565014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CYP1B1 Polyclonal Antibody (HA565014)

概述

图片

参考文献

产品说明书

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