Anti-Human POLG Polyclonal Antibody (HX196014)

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100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HX196014

品牌

abinScience

描述

Anti-Human POLG Polyclonal Antibody (HX196014) is a rabbit polyclonal antibody detecting POLG. Suitable for Human, Rat, and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human POLG (Trp1041-Pro1239).

靶标

3'-5' exodeoxyribonuclease, 5'-deoxyribose-phosphate lyase, DNA polymerase subunit gamma-1, EC:2.7.7.7, EC:3.1.11.-, EC:4.2.99.-, MDP1, Mitochondrial DNA polymerase catalytic subunit, POLG, POLG1, POLGA, PolG-alpha

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P54098

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

DNA polymerase subunit gamma-1 (POLG) is a ~139 kDa protein. Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site.

1. Johnson, AA. et al. (2001) The Journal of biological chemistry 276, 38090-6. PMID: 11477093

2. Ponamarev, MV. et al. (2002) The Journal of biological chemistry 277, 15225-8. PMID: 11897778

3. Luoma, PT. et al. (2005) Human molecular genetics 14, 1907-20. PMID: 15917273

4. Lee, YS. et al. (2009) Cell 139, 312-24. PMID: 19837034

5. Graves, SW. et al. (1998) Biochemistry 37, 6050-8. PMID: 9558343

6. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

Note

For research use only

图片

参考文献

产品说明书

Anti-Human POLG Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLG Polyclonal Antibody (HX196014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human POLG Polyclonal Antibody (HX196014)

概述

图片

参考文献

产品说明书

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