Anti-Human GEN1 Polyclonal Antibody (HD546014)

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概述
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参考文献
产品说明书

概述

货号

HD546014

品牌

abinScience

描述

Anti-Human GEN1 Polyclonal Antibody (HD546014) is a rabbit polyclonal antibody detecting GEN1. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human GEN1 (Met1-Pro473).

靶标

EC:3.1.-.-, Flap endonuclease GEN homolog 1, GEN1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q17RS7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Flap endonuclease GEN homolog 1 (GEN1) is a ~102 kDa protein. Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction.

1. Ip, SC. et al. (2008) Nature 456, 357-61. PMID: 19020614

2. Lee, SH. et al. (2015) eLife 4. PMID: 26682650

3. Chan, YW. et al. (2015) Nucleic acids research 43, 10882-92. PMID: 26578604

4. Shah Punatar, R. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 443-450. PMID: 28049850

Note

For research use only

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参考文献

产品说明书

Anti-Human GEN1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GEN1 Polyclonal Antibody (HD546014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human GEN1 Polyclonal Antibody (HD546014)

概述

图片

参考文献

产品说明书

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