Anti-Human TRIM22 Polyclonal Antibody (HC134014)

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规格：

50ug
100ug

数量：

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概述
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产品说明书

概述

货号

HC134014

品牌

abinScience

描述

Anti-Human TRIM22 Polyclonal Antibody (HC134014) is a rabbit polyclonal antibody detecting TRIM22. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TRIM22 (Ser317-Ser498).

靶标

50 kDa-stimulated trans-acting factor, E3 ubiquitin-protein ligase TRIM22, EC:2.3.2.27, RING finger protein 94, RING-type E3 ubiquitin transferase TRIM22, RNF94, STAF50, Staf-50, TRIM22, Tripartite motif-containing protein 22

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q8IYM9

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

E3 ubiquitin-protein ligase TRIM22 is a ~56 kDa protein. Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity. Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A. Also acts by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation. Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter.

1. Yang, C. et al. (2016) Cellular & molecular immunology 13, 94-102. PMID: 25683609

2. Inoue, A. et al. (2022) Biochimica et biophysica acta. Molecular cell research 1869, 119318. PMID: 35777501

3. Zu, S. et al. (2022) Cell & bioscience 12, 139. PMID: 36042495

4. Turrini, F. et al. (2015) Retrovirology 12, 104. PMID: 26683615

5. Fei, X. et al. (2022) Molecular therapy oncolytics 26, 413-428. PMID: 36159777

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-TRIM22 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: K562 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: Mouse thymus lysate
Lane 5: Mouse lung lysate

参考文献

产品说明书

Anti-Human TRIM22 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM22 Polyclonal Antibody (HC134014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM22 Polyclonal Antibody (HC134014)

概述

图片

参考文献

产品说明书

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