| 背景 | Pumilio homolog 1 (PUM1) is a ~126 kDa protein. Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. 1. Gupta, YK. et al. (2008) Structure (London, England : 1993) 16, 549-57. PMID: 183287182. Lu, G. et al. (2011) Structure (London, England : 1993) 19, 361-7. PMID: 213971873. Filipovska, A. et al. (2011) Nature chemical biology 7, 425-7. PMID: 215724254. Dong, S. et al. (2011) The Journal of biological chemistry 286, 26732-42. PMID: 216536945. Van Etten, J. et al. (2012) The Journal of biological chemistry 287, 36370-83. PMID: 229552767. Kedde, M. et al. (2010) Nature cell biology 12, 1014-20. PMID: 208183879. Miles, WO. et al. (2012) Genes & development 26, 356-68. PMID: 22345517 |
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