| 靶标 | Importin subunit beta-1, Importin-90, KPNB1, Karyopherin subunit beta-1, NTF97, Nuclear factor p97, PTAC97, Pore targeting complex 97 kDa subunit |
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| 背景 | Importin subunit beta-1 (KPNB1) is a ~97 kDa protein. Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. 1. Jäkel, S. et al. (1999) The EMBO journal 18, 2411-23. PMID: 102281562. Dean, KA. et al. (2001) Journal of cell science 114, 3479-85. PMID: 116826073. White, WO. et al. (2002) Journal of cellular biochemistry 85, 42-53. PMID: 118918494. Mingot, JM. et al. (2009) Journal of cell science 122, 1452-60. PMID: 193868975. Pawłowski, R. et al. (2010) The EMBO journal 29, 3448-58. PMID: 208183366. Choi, S. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1050-60. PMID: 246996497. Chi, NC. et al. (1995) The Journal of cell biology 130, 265-74. PMID: 76156308. Jäkel, S. et al. (1998) The EMBO journal 17, 4491-502. PMID: 9687515 |
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