Anti-Human UNG Polyclonal Antibody (HY404014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HY404014

品牌

abinScience

描述

Anti-Human UNG Polyclonal Antibody (HY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human UNG (Ala45-Leu313).

靶标

DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P13051

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596

2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827

3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234

4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144

5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300

6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285

7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624

8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759

Note

For research use only

图片

参考文献

产品说明书

Anti-Human UNG Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UNG Polyclonal Antibody (HY404014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UNG Polyclonal Antibody (HY404014)

概述

图片

参考文献

产品说明书

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