Anti-Human SPART Polyclonal Antibody (HC761014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC761014

品牌

abinScience

描述

Anti-Human SPART Polyclonal Antibody (HC761014) is a rabbit polyclonal antibody detecting SPART. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human SPART (Gly198-Gln336).

靶标

KIAA0610, SPART, SPG20, Spartin, Spastic paraplegia 20 protein, TAHCCP1, Trans-activated by hepatitis C virus core protein 1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q8N0X7

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Spartin is a ~72 kDa protein. Lipophagy receptor that plays an important role in lipid droplet (LD) turnover in motor neurons. Localizes to LDs and interacts with components of the autophagy machinery, such as MAP1LC3A/C proteins to deliver LDs to autophagosomes for degradation via lipophagy. Lipid transfer protein required for lipid droplet degradation, including by lipophagy. Can bind and transfer all lipid species found in lipid droplets, from phospholipids to triglycerides and sterol esters but the direction of lipid transfer by spartin and its cargos are unknown. May be implicated in endosomal trafficking, or microtubule dynamics, or both.

1. Chung, J. et al. (2023) Nature cell biology 25, 1101-1110. PMID: 37443287

2. Wan, N. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2314093121. PMID: 38190532

3. Renvoisé, B. et al. (2010) Molecular biology of the cell 21, 3293-303. PMID: 20719964

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-SPART polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: PANC1 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: K562 cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse heart lysate
Lane 8: Rat heart lysate
Lane 9: Mouse brain lysate
Lane 10: Rat brain lysate
Lane11: Mouse spleen lysate

参考文献

产品说明书

Anti-Human SPART Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SPART Polyclonal Antibody (HC761014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human SPART Polyclonal Antibody (HC761014)

概述

图片

参考文献

产品说明书

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