Anti-LCMV RdRp/Protein L Polyclonal Antibody (VK147014)

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概述
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参考文献
产品说明书

概述

货号

VK147014

品牌

abinScience

描述

Anti-LCMV RdRp/Protein L Polyclonal Antibody (VK147014) is a rabbit polyclonal antibody detecting Protein L. Suitable for Lymphocytic choriomeningitis virus.

种属反应性

Lymphocytic choriomeningitis virus (LCMV)

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant LCMV RdRp/Protein L (Asp2-Lys196).

靶标

EC:2.7.7.48, EC:3.1.-.-, L, Large structural protein, Protein L, RNA-directed RNA polymerase L, Replicase, Transcriptase, cap-snatching endonuclease

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P14240

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

RNA-directed RNA polymerase L (RdRp/Protein L) is a ~254 kDa protein. RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped.

1. Liu, L. et al. (2023) Protein & cell 14, 703-707. PMID: 37038286

Note

For research use only

图片

参考文献

产品说明书

Anti-LCMV RdRp/Protein L Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-LCMV RdRp/Protein L Polyclonal Antibody (VK147014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-LCMV RdRp/Protein L Polyclonal Antibody (VK147014)

概述

图片

参考文献

产品说明书

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