Anti-Mouse PPIA Polyclonal Antibody (MX128014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

MX128014

品牌

abinScience

描述

Anti-Mouse PPIA Polyclonal Antibody (MX128014) is a rabbit polyclonal antibody detecting Ppia. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Mouse PPIA (Met1-Leu164).

靶标

Cyclophilin A, Cyclosporin A-binding protein, EC:5.2.1.8, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Peptidyl-prolyl cis-trans isomerase A, N-terminally processed, Ppia, Rotamase A, SP18

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P17742

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Peptidyl-prolyl cis-trans isomerase A (PPIA) is a ~17 kDa protein. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a pro-inflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2.

1. Wang, L. et al. (2014) Thrombosis and haemostasis 111, 873-82. PMID: 24429998

2. Lauranzano, E. et al. (2015) Brain : a journal of neurology 138, 974-91. PMID: 25678563

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-PPIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: K562 cell lysate
Lane 4: Raji cell lysate
Lane 5: Jurkat cell lysate
Lane 6: PC-12 cell lysate
Lane 7: 293T cell lysate
Lane 8: Mouse brain lysate
Lane 9: Rat brain lysate

参考文献

产品说明书

Anti-Mouse PPIA Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse PPIA Polyclonal Antibody (MX128014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Mouse PPIA Polyclonal Antibody (MX128014)

概述

图片

参考文献

产品说明书

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