Anti-Human TRIM65 Polyclonal Antibody (HD347014)

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概述
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参考文献
产品说明书

概述

货号

HD347014

品牌

abinScience

描述

Anti-Human TRIM65 Polyclonal Antibody (HD347014) is a rabbit polyclonal antibody detecting TRIM65. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TRIM65 (Val319-Ser517).

靶标

E3 ubiquitin-protein ligase TRIM65, EC:2.3.2.27, TRIM65, Tripartite motif-containing protein 65

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q6PJ69

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

E3 ubiquitin-protein ligase TRIM65 is a ~57 kDa protein. E3 ubiquitin ligase that plays a role in several processes including innate immnity, autophagy or inflammation. Negatively regulates miRNAs by modulating the ubiquitination and stability of TNRC6A, a protein involved in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs. This ubiquitination results in the suppressed expression of miR-138-5p leading to increased autophagy. Upon enteroviral infection, promotes 'Lys-63'-mediated ubiquitination activation of IFIH1/MDA5 leading to innate signaling cascade. Mechanistically, selectively recognizes MDA5 filaments that occur on dsRNAs.

1. Meng, J. et al. (2017) Cell death & disease 8, e2866. PMID: 28594402

2. Tang, T. et al. (2021) Frontiers in immunology 12, 741839. PMID: 34512673

3. Li, S. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 6970-5. PMID: 24778252

4. Pan, X. et al. (2019) Cell death & disease 10, 429. PMID: 31160576

5. Kato, K. et al. (2021) Molecular cell 81, 599-613.e8. PMID: 33373584

6. Li, Y. et al. (2020) Journal of molecular cell biology 12, 190-201. PMID: 31310649

Note

For research use only

图片

参考文献

产品说明书

Anti-Human TRIM65 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM65 Polyclonal Antibody (HD347014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRIM65 Polyclonal Antibody (HD347014)

概述

图片

参考文献

产品说明书

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