Anti-Human WIPI1 Polyclonal Antibody (HD292014)

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50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HD292014

品牌

abinScience

描述

Anti-Human WIPI1 Polyclonal Antibody (HD292014) is a rabbit polyclonal antibody detecting WIPI1. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human WIPI1 (Leu17-Leu360).

靶标

Atg18 protein homolog, WD repeat domain phosphoinositide-interacting protein 1, WD40 repeat protein interacting with phosphoinositides of 49 kDa, WIPI 49 kDa, WIPI-1, WIPI1, WIPI49

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q5MNZ9

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1) is a ~48 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Plays an important role in starvation- and calcium-mediated autophagy, as well as in mitophagy. Functions downstream of the ULK1 and PI3-kinases that produce phosphatidylinositol 3-phosphate (PtdIns3P) on membranes of the endoplasmic reticulum once activated. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and maybe other phosphoinositides including PtdIns3,5P2 and PtdIns5P, and is recruited to phagophore assembly sites at the endoplasmic reticulum membranes. There, it assists WIPI2 in the recruitment of ATG12-ATG5-ATG16L1, a complex that directly controls the elongation of the nascent autophagosomal membrane.

1. Proikas-Cezanne, T. et al. (2004) Oncogene 23, 9314-25. PMID: 15602573

2. Grotemeier, A. et al. (2010) Cellular signalling 22, 914-25. PMID: 20114074

3. Geeraert, C. et al. (2010) The Journal of biological chemistry 285, 24184-94. PMID: 20484055

4. Itakura, E. et al. (2010) Autophagy 6, 764-76. PMID: 20639694

5. Gaugel, A. et al. (2012) Journal of molecular signaling 7, 16. PMID: 23088497

6. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066

7. Maeda, S. et al. (2019) eLife 8. PMID: 31271352

8. Kojima, W. et al. (2021) Autophagy 17, 2011-2036. PMID: 33499712

Note

For research use only

图片

参考文献

产品说明书

Anti-Human WIPI1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human WIPI1 Polyclonal Antibody (HD292014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human WIPI1 Polyclonal Antibody (HD292014)

概述

图片

参考文献

产品说明书

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