Anti-Human TRMT5 Polyclonal Antibody (HD270014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HD270014

品牌

abinScience

描述

Anti-Human TRMT5 Polyclonal Antibody (HD270014) is a rabbit polyclonal antibody detecting TRMT5. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human TRMT5 (Thr65-Lys475).

靶标

EC:2.1.1.228, KIAA1393, M1G-methyltransferase, TRM5, TRMT5, tRNA (guanine(37)-N(1))-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5 homolog

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q32P41

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

tRNA (guanine(37)-N(1))-methyltransferase (TRMT5) is a ~58 kDa protein. Involved in mitochondrial tRNA methylation. Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

1. Powell, CA. et al. (2015) American journal of human genetics 97, 319-28. PMID: 26189817

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-TRMT5 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

参考文献

产品说明书

Anti-Human TRMT5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRMT5 Polyclonal Antibody (HD270014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human TRMT5 Polyclonal Antibody (HD270014)

概述

图片

参考文献

产品说明书

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