Anti-Human ORF2p Polyclonal Antibody (HD130014)

价格:

规格：

50ug
100ug

数量：

联系我们

一键复制产品信息

概述
图片
参考文献
产品说明书

概述

货号

HD130014

品牌

abinScience

描述

Anti-Human ORF2p Polyclonal Antibody (HD130014) is a rabbit polyclonal antibody detecting ORF2p. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ORF2p (Met1-Lys240).

靶标

EC:2.7.7.49, EC:3.1.21.-, Endonuclease, LINE-1 retrotransposable element ORF2 protein, ORF2p, Reverse transcriptase

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O00370

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

LINE-1 retrotransposable element ORF2 protein (ORF2p) is a ~149 kDa protein. Has reverse transcriptase activity required for target-primed reverse transcription of the LINE-1 element mRNA, a crucial step in LINE-1 retrotransposition. Selectively binds and reversely transcribes RNA with a poly(A) tail consisting of at least 20 adenosines. Also has endonuclease activity that allows the introduction of nicks in the chromosomal target DNA. Cleaves DNA in AT-rich regions between a 5' stretch of purines and a 3' stretch of pyrimidines, corresponding to the sites of LINE-1 integration in the genome. Conformational properties of the target DNA sequence rather than specific nucleotides are key determinants of the ORF2p capacity for sequence-specific DNA recognition.

1. Thawani, A. et al. (2024) Nature 626, 186-193. PMID: 38096901

2. Baldwin, ET. et al. (2024) Nature 626, 194-206. PMID: 38096902

3. Dombroski, BA. et al. (1994) Molecular and cellular biology 14, 4485-92. PMID: 7516468

4. Sassaman, DM. et al. (1997) Nature genetics 16, 37-43. PMID: 9140393

5. Repanas, K. et al. (2007) Nucleic acids research 35, 4914-26. PMID: 17626046

6. Miller, I. et al. (2021) Nucleic acids research 49, 11350-11366. PMID: 34554261

7. Feng, Q. et al. (1996) Cell 87, 905-16. PMID: 8945517

Note

For research use only

图片

参考文献

产品说明书

Anti-Human ORF2p Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ORF2p Polyclonal Antibody (HD130014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ORF2p Polyclonal Antibody (HD130014)

概述

图片

参考文献

产品说明书

相关产品推荐

获取报价