Anti-Human ETF1 Polyclonal Antibody (HD118014)

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100ug

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概述
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参考文献
产品说明书

概述

货号

HD118014

品牌

abinScience

描述

Anti-Human ETF1 Polyclonal Antibody (HD118014) is a rabbit polyclonal antibody detecting ETF1. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ETF1 (Met1-Ser257).

靶标

ERF1, ETF1, Eukaryotic peptide chain release factor subunit 1, Eukaryotic release factor 1, Protein Cl1, RF1, SUP45L1, TB3-1, eRF1

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P62495

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Eukaryotic peptide chain release factor subunit 1 (ETF1) is a ~49 kDa protein. Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons. The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site. ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA. Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons.

1. Song, H. et al. (2000) Cell 100, 311-21. PMID: 10676813

2. Alkalaeva, EZ. et al. (2006) Cell 125, 1125-36. PMID: 16777602

3. Feng, T. et al. (2014) Molecular cell 53, 645-54. PMID: 24486019

4. Brown, A. et al. (2015) Nature 524, 493-496. PMID: 26245381

5. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

6. Oltion, K. et al. (2023) Cell 186, 346-362.e17. PMID: 36638793

7. Frolova, L. et al. (1994) Nature 372, 701-3. PMID: 7990965

8. Yamashita, A. et al. (2009) Genes & development 23, 1091-105. PMID: 19417104

9. Wang, X. et al. (2019) Cell 176, 625-635.e14. PMID: 30682371

Note

For research use only

图片

参考文献

产品说明书

Anti-Human ETF1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ETF1 Polyclonal Antibody (HD118014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ETF1 Polyclonal Antibody (HD118014)

概述

图片

参考文献

产品说明书

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