Anti-Human WDR4 Polyclonal Antibody (HD097014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HD097014

品牌

abinScience

描述

Anti-Human WDR4 Polyclonal Antibody (HD097014) is a rabbit polyclonal antibody detecting WDR4. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human WDR4 (Met1-Cys412).

靶标

Protein Wuho homolog, WD repeat-containing protein 4, WDR4, hWH, tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P57081

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 is a ~45 kDa protein. Non-catalytic component of the METTL1-WDR4 methyltransferase complex required for the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs). In the METTL1-WDR4 methyltransferase complex, WDR4 acts as a scaffold for tRNA-binding. Required for the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop. M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay. Also required for the formation of N(7)-methylguanine at internal sites in a subset of mRNAs.

1. Alexandrov, A. et al. (2002) RNA (New York, N.Y.) 8, 1253-66. PMID: 12403464

2. Pandolfini, L. et al. (2019) Molecular cell 74, 1278-1290.e9. PMID: 31031083

3. Zhang, LS. et al. (2019) Molecular cell 74, 1304-1316.e8. PMID: 31031084

4. Ruiz-Arroyo, VM. et al. (2023) Nature 613, 383-390. PMID: 36599982

5. Li, J. et al. (2023) Nature 613, 391-397. PMID: 36599985

6. Jin, X. et al. (2023) Cell discovery 9, 65. PMID: 37369656

7. Dai, Z. et al. (2021) Molecular cell 81, 3339-3355.e8. PMID: 34352206

8. Orellana, EA. et al. (2021) Molecular cell 81, 3323-3338.e14. PMID: 34352207

9. Zhao, Z. et al. (2023) Cell 186, 3208-3226.e27. PMID: 37379838

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-WDR4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Rat kidney lysate

参考文献

产品说明书

Anti-Human WDR4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human WDR4 Polyclonal Antibody (HD097014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human WDR4 Polyclonal Antibody (HD097014)

概述

图片

参考文献

产品说明书

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