Anti-Human UBR5 Polyclonal Antibody (HC972014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC972014

品牌

abinScience

描述

Anti-Human UBR5 Polyclonal Antibody (HC972014) is a rabbit polyclonal antibody detecting UBR5. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human UBR5 (Pro640-Ala870).

靶标

E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EC:2.3.2.26, EDD, EDD1, HYD, Hyperplastic discs protein homolog, KIAA0896, Progestin-induced protein, UBR5, hHYD

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

O95071

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

E3 ubiquitin-protein ligase UBR5 is a ~309 kDa protein. E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm and nucleus. Mainly acts as a ubiquitin chain elongator that extends pre-ubiquitinated substrates. Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation. Recognizes type-1 N-degrons, containing positively charged amino acids (Arg, Lys and His). Together with UBR4, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR5 is probably branching multiple 'Lys-48'-linked chains of substrates initially modified with mixed conjugates by UBR4.

1. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

2. Schukur, L. et al. (2020) Scientific reports 10, 20044. PMID: 33208877

3. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846

4. Mark, KG. et al. (2023) Cell 186, 3460-3475.e23. PMID: 37478862

5. Hodáková, Z. et al. (2023) The EMBO journal 42, e113348. PMID: 37409633

6. Ohtake, F. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1401-E1408. PMID: 29378950

10. Jiang, W. et al. (2011) Molecular cell 43, 33-44. PMID: 21726808

Note

For research use only

图片

参考文献

产品说明书

Anti-Human UBR5 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UBR5 Polyclonal Antibody (HC972014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human UBR5 Polyclonal Antibody (HC972014)

概述

图片

参考文献

产品说明书

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