Anti-Human NEDD8 Polyclonal Antibody (HC254014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC254014

品牌

abinScience

描述

Anti-Human NEDD8 Polyclonal Antibody (HC254014) is a rabbit polyclonal antibody detecting NEDD8. Suitable for Rat, Mouse, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NEDD8 (Leu2-Gln81).

靶标

NEDD-8, NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, Ubiquitin-like protein NEDD8

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q15843

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Ubiquitin-like protein NEDD8 is a ~9 kDa protein. Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.

1. Liakopoulos, D. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 5510-5. PMID: 10318914

2. Hori, T. et al. (1999) Oncogene 18, 6829-34. PMID: 10597293

3. Amir, RE. et al. (2002) The Journal of biological chemistry 277, 23253-9. PMID: 11953428

4. Walden, H. et al. (2003) Molecular cell 12, 1427-37. PMID: 14690597

5. Xirodimas, DP. et al. (2004) Cell 118, 83-97. PMID: 15242646

6. Osaka, F. et al. (1998) Genes & development 12, 2263-8. PMID: 9694792

7. Horn-Ghetko, D. et al. (2024) Nature structural & molecular biology 31, 1083-1094. PMID: 38605244

8. Kokic, G. et al. (2024) Nature structural & molecular biology 31, 536-547. PMID: 38316879

Note

For research use only

图片

参考文献

产品说明书

Anti-Human NEDD8 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NEDD8 Polyclonal Antibody (HC254014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NEDD8 Polyclonal Antibody (HC254014)

概述

图片

参考文献

产品说明书

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