Anti-Human NASP Polyclonal Antibody (HC209014)

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规格：

50ug
100ug

数量：

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概述
图片
参考文献
产品说明书

概述

货号

HC209014

品牌

abinScience

描述

Anti-Human NASP Polyclonal Antibody (HC209014) is a rabbit polyclonal antibody detecting NASP. Suitable for Mouse, Human, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse, Rat

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human NASP (Gly513-Gly681).

靶标

NASP, Nuclear autoantigenic sperm protein

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P49321

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Nuclear autoantigenic sperm protein (NASP) is a ~85 kDa protein. Component of the histone chaperone network. Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy. Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

1. Cook, AJ. et al. (2011) Molecular cell 44, 918-27. PMID: 22195965

Note

For research use only

图片

Western blot

Western blot analysis was performed using anti-NASP polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate
Lane 2: 293T cell lysate
Lane 3: HL-60 cell lysate

参考文献

产品说明书

Anti-Human NASP Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NASP Polyclonal Antibody (HC209014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human NASP Polyclonal Antibody (HC209014)

概述

图片

参考文献

产品说明书

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