Anti-Human MCM4 Polyclonal Antibody (HC208014)

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50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC208014

品牌

abinScience

描述

Anti-Human MCM4 Polyclonal Antibody (HC208014) is a rabbit polyclonal antibody detecting MCM4. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human MCM4 (Asn275-Asp775).

靶标

CDC21, CDC21 homolog, DNA replication licensing factor MCM4, EC:3.6.4.12, MCM4, P1-CDC21

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P33991

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

DNA replication licensing factor MCM4 is a ~96 kDa protein. Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

1. Tsuji, T. et al. (2006) Molecular biology of the cell 17, 4459-72. PMID: 16899510

2. Ishimi, Y. et al. (2015) Journal of biochemistry 157, 561-9. PMID: 25661590

3. Rzechorzek, NJ. et al. (2020) Nucleic acids research 48, 6980-6995. PMID: 32453425

4. Jones, ML. et al. (2021) The EMBO journal 40, e108819. PMID: 34694004

5. Jenkyn-Bedford, M. et al. (2021) Nature 600, 743-747. PMID: 34700328

6. Baris, Y. et al. (2022) Nature 606, 204-210. PMID: 35585232

7. Ishimi, Y. (1997) The Journal of biological chemistry 272, 24508-13. PMID: 9305914

Note

For research use only

图片

参考文献

产品说明书

Anti-Human MCM4 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MCM4 Polyclonal Antibody (HC208014)

概述

图片

参考文献

产品说明书

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重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human MCM4 Polyclonal Antibody (HC208014)

概述

图片

参考文献

产品说明书

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