Anti-Human CYP7A1 Polyclonal Antibody (HC204014)

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100ug

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概述
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概述

货号

HC204014

品牌

abinScience

描述

Anti-Human CYP7A1 Polyclonal Antibody (HC204014) is a rabbit polyclonal antibody detecting CYP7A1. Suitable for Human.

种属反应性

Human

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human CYP7A1 (Gly43-Asp397).

靶标

24-hydroxycholesterol 7-alpha-hydroxylase, CYP7, CYP7A1, CYPVII, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, Cytochrome P450 7A1, EC:1.14.14.23, EC:1.14.14.26

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

P22680

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Cytochrome P450 7A1 (CYP7A1) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol.

1. Norlin, M. et al. (2000) Journal of lipid research 41, 1629-39. PMID: 11013305

2. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124

3. Li, T. et al. (2010) Journal of lipid research 51, 832-42. PMID: 19965590

4. Shinkyo, R. et al. (2011) The Journal of biological chemistry 286, 33021-8. PMID: 21813643

5. Noshiro, M. et al. (1990) FEBS letters 268, 137-40. PMID: 2384150

Note

For research use only

图片

参考文献

产品说明书

Anti-Human CYP7A1 Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CYP7A1 Polyclonal Antibody (HC204014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human CYP7A1 Polyclonal Antibody (HC204014)

概述

图片

参考文献

产品说明书

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