Anti-Human ISCU Polyclonal Antibody (HC184014)

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规格：

50ug
100ug

数量：

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概述
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参考文献
产品说明书

概述

货号

HC184014

品牌

abinScience

描述

Anti-Human ISCU Polyclonal Antibody (HC184014) is a rabbit polyclonal antibody detecting ISCU. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

种属反应性

Human, Mouse

应用

ELISA, IHC, WB

宿主

Rabbit

克隆类型

Polyclonal

同种型

IgG

免疫原

E. coli - derived recombinant Human ISCU (His36-Lys167).

靶标

ISCU, Iron-sulfur cluster assembly enzyme ISCU, NIFUN, NifU-like N-terminal domain-containing protein, NifU-like protein

内毒素水平

Please contact with the lab for this information.

纯化方式

Purified by antigen affinity column.

Accession号

Q9H1K1

状态

Liquid

保存溶液

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

产品使用信息

应用方法	稀释比例
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

背景

Iron-sulfur cluster assembly enzyme ISCU is a ~17 kDa protein. Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled. The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable).

1. Freibert, SA. et al. (2021) Nature communications 12, 6902. PMID: 34824239

2. Bridwell-Rabb, J. et al. (2014) Biochemistry 53, 4904-13. PMID: 24971490

3. Cai, K. et al. (2018) Journal of inorganic biochemistry 183, 107-116. PMID: 29576242

4. Fox, NG. et al. (2018) Biochimie 152, 211-218. PMID: 30031876

5. Cai, K. et al. (2013) The Journal of biological chemistry 288, 28755-70. PMID: 23940031

Note

For research use only

图片

参考文献

产品说明书

Anti-Human ISCU Polyclonal Antibody.pdf

公式

质量 (g) = 浓度 (mol/L) × 体积 (L) × 分子量 (g/mol)

填写 质量、浓度、体积中的任意 2 项 + 分子量，自动计算未知值。

质量

浓度

体积

分子量 *

g/mol

公式

C₁ × V₁ = C₂ × V₂

填写 4 项中的任意 3 项，自动计算未知值。

母液

C₁ (起始浓度)

V₁ (起始体积)

工作液

C₂ (终浓度)

V₂ (终体积)

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ISCU Polyclonal Antibody (HC184014)

概述

图片

参考文献

产品说明书

相关产品推荐

重组蛋白

抗体

试剂盒

实验相关试剂

Anti-Human ISCU Polyclonal Antibody (HC184014)

概述

图片

参考文献

产品说明书

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